Heparinoid-active two sulfated polysaccharides isolated from marine green algae Monostroma nitidum |
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Authors: | Wen-Jun Mao Fang Fang Hong-Yan Li Xiao-Hui Qi Hai-Hong Sun Yin Chen Shou-Dong Guo |
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Institution: | aInstitute of Marine Drug and Food, Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, China |
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Abstract: | Two sulfated polysaccharides WF1 and WF3 were isolated from marine green algae Monostroma nitidum, and their structural characteristics were determined. Anticoagulant activities of WF1 and WF3 were evaluated by assays of the activated partial thromboplastin time (APTT), thrombin time (TT), prothrombin time (PT), antithrombin and anticoagulation factor Xa activities. The results showed that WF1 and WF3 had similar high contents of rhamnose, whereas their sulfate contents, sulfation positions, molecular sizes and linkage patterns of rhamnose residues were different. The bioassay results demonstrated that WF1 and WF3 had high anticoagulant activities, and were potent thrombin inhibitors mediated by heparin cofactor II, especially WF3. They also hastened thrombin and coagulation factor Xa inhibition by potentiating antithrombin III, but at a lower effectiveness. The differences of anticoagulant activities between WF1 and WF3 were directly due to their structural features discrepancy. |
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Keywords: | Monostroma nitidum Sulfated polysaccharide Chemical characteristic Activated partial thromboplastin time Thrombin time Heparin cofactor II |
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