A candidate gene for lignin composition in Eucalyptus: cinnamoyl-CoA reductase (CCR) |
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Authors: | Eric Mandrou Paulo Ricardo Gherardi Hein Emilie Villar Philippe Vigneron Christophe Plomion and Jean-Marc Gion |
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Institution: | (1) Centre de Recherche Vallourec, CEV, Route de Leval BP 17, Aulnoye Aymeries, France;(2) CIRAD, UMR AGAP, Equipe G?n?tique et Am?lioration des Esp?ces P?rennes: Mod?les For?t & Palmier, Campus International de Baillarguet TA A-39/C, Montpellier cedex 5, France;(3) INRA, UMR 1202 BIOGECO, 69 Route d’Arcachon, 33612 Cestas Pierroton, France;(4) CIRAD, UPR40 Production and Processing of Tropical Woods, 73 rue J-F Breton TA B?40/16, Montpellier, France;(5) CRDPI, BP 1291, Pointe-Noire, R?publique du Congo;(6) Universit? Bordeaux 1, 69 Route d’Arcachon, Cestas Pierroton, France; |
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Abstract: | Lignin content and composition are considered as mandatory traits of eucalyptus breeding programs, especially for pulp, paper,
and bioenergy production. In this article, we used 33 Eucalyptus urophylla full-sib families of an 8 × 8 factorial design to provide estimates of genetic parameters for lignin- and growth-related
traits. Secondly, from the sequencing of the 16 unrelated founders, we described the nucleotide and haplotype variability
of cinnamoyl-CoA reductase (CCR), a candidate gene for lignin-related traits encoding the cinnamoyl-CoA reductase. Finally, we tested the association between
CCR polymorphisms and trait variation using a mixed linear model. A high value of narrow sense heritability was obtained for
lignin content (h2 = 0.85) and S/G ratio (h2 = 0.62) indicating that these traits are under strong genetic control. High levels of nucleotide (θπ = 0.0131) and haplotype (Hd = 0.958) diversity were detected for CCR. From an initial set of 152 biallelic single nucleotide polymorphisms (SNPs), a subset of 65 nonredundant loci was selected.
Three intronic SNPs were found to be associated to the variation of S/G ratio after multiple testing correction. In the line
of what has been obtained in forest trees, these SNPs explained between 2.45% and 2.87% of the genetic variance of the trait.
This study demonstrates the interest of the candidate gene approach for quantitative trait nucleotide detection in Eucalyptus and paves the way to gene assisted selection of lignin composition in E. urophylla. |
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