HSP90s are required for NLR immune receptor accumulation in Arabidopsis |
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| Authors: | Shuai Huang Jacqueline Monaghan Xionghui Zhong Ling Lin Tongjun Sun Oliver Xiaoou Dong Xin Li |
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| Affiliation: | 1. Michael Smith Laboratories, University of British Columbia, , Vancouver, BC, V6T 1Z4 Canada;2. Department of Botany, University of British Columbia, , Vancouver, BC, V6T 1Z4 Canada;3. Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, , Beijing, 100083 China;4. Institute of Crop Protection, Jiangsu Agricultural Academy, , Nanjing, 210023 China |
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| Abstract: | Heat shock proteins (HSPs) serve as molecular chaperones for diverse client proteins in many biological processes. In plant immunity, cytosolic HSP90s participate in the assembly, stability control and/or activation of immune receptor complexes. In this paper we report that in addition to the well‐established positive roles that HSP90 isoforms play in plant immunity, they are also involved in the negative regulation of immune receptor accumulation. Point mutations in two HSP90 genes, HSP90.2 and HSP90.3, were identified from a forward genetic screen designed to isolate mutants with enhanced disease resistance. We found that specific mutations in HSP90.2 and HSP90.3 lead to heightened accumulation of immune receptors, including SNC1, RPS2 and RPS4. HSP90s may assist SGT1 in the formation of SCF E3 ubiquitin ligase complexes that target immune receptors for degradation. Such regulation is critical for maintaining appropriate levels of immune receptor proteins to avoid autoimmunity. |
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| Keywords: | HSP90 NLR resistance protein turnover SKP1– Cullin 1– F‐Box SNC1 RPS2 RPS4 SGT1 plant immunity Arabidopsis |
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