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Plant regeneration from callus cultures of Psoralea corylifolia Linn
Authors:C Saxena  SK Palai  S Samantaray  GR Rout  P Das
Institution:(1) Plant Biotechnology Division, Plant Tissue Culture Laboratory, Regional Plant Resource Centre, Bhubaneswar, 751 015, India;(2) Plant Physiology and Biochemistry Laboratory, Regional Plant Resource Centre, Bhubaneswar, 751 015, India
Abstract:Plantlet regeneration via organogenesis was achieved in callus cultures derived form mature leaves, stems and leaves, petioles and roots of young seedling of Psoralea corylifolia on Murashige and Skoog medium supplemented with 2.5–3.0 mg L-1 BA, 1.0 mg L-1 NAA and 3% (w/v) sucrose. The rate of shoot bud regeneration was positively correlated with the concentration of hormones in the nutrient media. Shoot buds regenerated more readily from juvenile explants (seedling source) as compared to the mature explants. Addition of adenine sulphate (5 mg L-1) to the culture medium increased the growth of shoot buds. Optimum responses were obtained in hypocotyl and leaf explants using NAA in combination with BA, the highest rate of shoot bud regeneration being in hypocotyl explants. Rooting was readily achieved on the differentiated shoots on MS basal media without growth regulators. Regenerated plantlets were successfully established in the greenhouse.
Keywords:BA  in vitro  medicinal plant  NAA  regeneration
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