Transgenic mimicry of pathogen attack stimulates growth and secondary metabolite accumulation |
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Authors: | Kuntal Chaudhuri Sudripta Das Moumita Bandyopadhyay Andreja Zalar Albert Kollmann Sumita Jha David Tepfer |
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Institution: | (1) Department of Botany, University of Calcutta, 35 Ballygunge Circular Road, Calcutta, 700019, India;(2) Present address: Department of Botany, Vivekananda College, 269 Diamond Harbour Road, Calcutta, 700063, India;(3) Present address: Department of Biotechnology, Tea Research Association, Tocklai, Jorhat, 785008, Assam, India;(4) Biologie de la Rhizosphère, Institut National de la Recherche Agronomique, Versailles Cedex, 78026, France |
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Abstract: | Plant secondary metabolites, including pharmaceuticals, flavorings and aromas, are often produced in response to stress. We
used chemical inducers of the pathogen defense response (jasmonic acid, salicylate, killed fungi, oligosaccharides and the
fungal elicitor protein, cryptogein) to increase metabolite and biomass production in transformed root cultures of the medicinal
plant, Withania somnifera, and the weed, Convolvulus sepium. In an effort to genetically mimic the observed effects of cryptogein, we employed Agrobacterium rhizogenes to insert a synthetic gene encoding cryptogein into the roots of C. sepium, W. somnifera and Tylophora tanakae. This genetic transformation was associated with stimulation in both secondary metabolite production and growth in the first
two species, and in growth in the third. In whole plants of Convolvulus arvensis and Arabidopsis thaliana, transformation with the cryptogein gene led, respectively, to increases in the calystegines and certain flavonoids. A similar
transgenic mimicry of pathogen attack was previously employed to stimulate resistance to the pathogen and abiotic stress.
In the present study of biochemical phenotype, we show that transgenic mimicry is correlated with increased secondary metabolite
production in transformed root cultures and whole plants. We propose that natural transformation with genes encoding the production
of microbial elicitors could influence interactions between plants and other organisms. |
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Keywords: | Cryptogein Flavonoids Withania Tylophora Convolvulus Calystegia |
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