Characterization of chromosomes and localization of the rDNA locus in the aye-aye (Daubentonia madagascariensis). |
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| Authors: | D A Tagle M Goodman D A Miller |
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| Institution: | Department of Molecular Biology and Genetics, Wayne State University School of Medicine, Detroit, MI 48201. |
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| Abstract: | The karyotype of a prosimian primate, the aye-aye (Daubentonia madagascariensis), is described. Results from a variety of staining methods (Q-, R-, G-, and C-banding, distamycin A/DAPI and methyl-green/DAPI) are reported. Sites of methylation were visualized using antibodies against 5-methylcytosine. Digestion of aye-aye fixed metaphase chromosomes with the restriction endonuclease HaeIII produced G-banding. No other restriction enzymes tested produced clear G- or C-banding patterns. Ag-staining of the nucleolar organizer regions (NORs) revealed the location of rDNA sites on the short arms of the smallest pairs of acrocentric chromosomes, 13p and 14p. |
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