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Purification and crystallization of insecticidal δ-endotoxin CryIIIB2 from Bacillus thuringiensis
Authors:Vivian Cody  Joseph R Luft  Erik Jensen  Walter Pangborn  Leigh English
Abstract:CryIIIB2, an insecticidal protein from Bacillus thuringiensis has been crystallized from 0.6 M NaBr and HEPES buffer at pH 7.0 and X-ray diffraction data collected on a native crystal to 2.4 Å. The insecticidal protein was obtained from a Bacillus thuringiensis (Bt) strain EG7231. Crystals of the endotoxin are orthorhombic, space group C2221, with unit cell dimensions of a = 122.44, b = and c = Å. A unit cell contains one molecule of the 67,000 Da endotoxin per asymmetric unit. © 1992 Wiley-Liss, Inc.
Keywords:Bacillus thuringiensis  insecticidal  δ  -endotoxin  crystallization
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