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Reversible dissociation of the plant golgi apparatus by brefeldin A
Authors:Be atrice Satiat-Jeunemaitre  Chris Hawes
Institution:Be´atrice Satiat-Jeunemaitre,Chris Hawes
Abstract:Summary— The effects of the drug Brefeldin A, shown to block the translocation of proteins between the endoplasmic reticulum and the Golgi apparatus in animal cells, were studied on different plant cell systems. In suspension culture cells and root tissues, the Golgi aparatus was affected by Brefeldin A treatments resulting in distortion and dissociation of the Golgi stacks, coupled with appearance of numerous vesicles in the cytoplasm. This process was reversible. Therefore, Brefeldin A provides a powerful tool with which to study Golgi dynamics and function in plant as well as in animal cells.
Keywords:Brefeldin A  vesicular traffic  Golgi apparatus  plant cells
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