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Development of an immobilization method of L-arabinose isomerase for industrial production of tagatose |
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| Authors: | Deok-Kun Oh Hye-Jung Kim Se-Ah Ryu Hoe-Jin Rho Pil Kim |
| Affiliation: | (1) Department of Bioscience and Biotechnology, Sejong University, Seoul, 143-747, Korea;(2) Basic Research Team, R&D Center, Tong Yang Confectionery Co., Seoul, 140-715, Korea |
| Abstract: | Of the immobilization methods tested, alginate beads treated with glutaraldehyde gave the most stable and economic method for immobilizing l-arabinose isomerase for the industrial production of tagatose. l-Arabinose isomerase immobilized in a packed-bed reactor produced an average of 30 g tagatose l–1 day–1 from 100 g galactose l–1 for 8 days. |
| Keywords: | alginate beads l-arabinose isomerase glutaraldehyde immobilization packed-bed reactor tagatose |
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