Efficient regeneration of <Emphasis Type="Italic">Sphagnum fallax</Emphasis> from isolated protoplasts |
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| Authors: | Amla?Batra Horst?Binding Susanne?Rasmussen Hansj?rg?Rudolph Email author" target="_blank">Georg?H?WaetzigEmail author |
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| Institution: | (1) Department of Botany, University of Rajasthan, Jaipur, India;(2) Institute of Botany, Christian-Albrechts-University, Kiel, Germany;(3) AgResearch, Grasslands Research Centre, Palmerston North, New Zealand;(4) Mucosal Immunology Group, First Department of Medicine, Kiel University Medical Center, Schittenhelmstrasse 12, 24105 Kiel, Germany |
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| Abstract: | Summary While the in vitro clonal propagation of peat mosses (Sphagnaceae) in bioreactors has been established since the late 1980s, it has never been possible to regenerate Sphagnum species from isolated protoplasts, which is a key step towards the production of closely defined genetically modified clones.
The present study describes an efficient protocol for protoplast isolation and regeneration of Sphagnum fallax. Protoplast survival rates of over 50% and regeneration rates of up to 20% were achieved by using excised capitulum buds
as starting material and by co-cultivating Sphagnum protoplasts with protoplasts from a chlorophyll-deficient Solanum hybrid clone. Besides the effects of nutrient components and differential osmotic readjustment of the regenerant cell clusters,
the interference of unique Sphagnum phenolics, sphagnum acid and hydroxybutenolide, with protoplast isolation efficiency is demonstrated. |
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| Keywords: | peat moss Sphagnum gametophyte regeneration protoplast isolation co-culture techniques |
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