Localization of lipolytic and esterolytic activities of Tyromyces sambuceus,a 4-decanolide-producing basidiomycete |
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Authors: | S. Hädrich-Meyer R. G. Berger |
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Affiliation: | (1) Institut für Lebensmittelchemie der Universität Hannover, Wunstorfer Strasse 14, D-30453 Hannover, Germany |
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Abstract: | Pellets of Tyromyces sambuceus were cultured in standard liquid media that contained a natural triacylglycerol as an additional carbon source. Parallel to a drop of pH below 2.0 and the formation of R(+)-4-decanolide, the oil droplets disappeared. Assays based on 3-naphthyl ester showed that most of the lipolytic and esterolytic enzyme activities were membrane-associated. The time course of enzyme activities after addition of castor oil suggested an inducibility of both membrane-bound and intracellular lipases, while only the intracellular esterases responded significantly. The results could contribute to improving the biotechnological generation of lipid-derived flavours and other compounds. |
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