Retention of a dialkylphosphatidylcholine in myelin and other membranes |
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Authors: | H Brockerhoff H M Wisniewski L C Lipton D S Deshmukh |
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Institution: | (1) Institute for Basic Research in Mental Retardation, 1050 Forest Hill Road, 10314 Staten Island, New York |
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Abstract: | We describe an attempt to incorporate a metabolically inert phospholipid analog into animal membranes, especially myelin, in vivo, with the view of eventual long-term membrane modification or membrane engineering. A sonicated suspension of a mixture of 14C] phosphatidylcholine and its dialkyl analog, 3H] tetradecyloctadecano(1)phosphocholine, was injected into the brain of weanling rats. Samples were counted of whole brain, myelin, liver, and carcass, at intervals from 1 to 63 days, and the composition of the extracted labeled lipid was determined by thin-layer chromatography. Both lipid labels were found to be cleared from the body at similar rates, but while phosphatidylcholine was metabolized within a day, with the label appearing mainly in the phosphatidylethanolamine fraction and in nonpolar lipids, the dialkylphosphatidylcholine remained intact, with retention in myelin of a small but almost constant amount for a month. Ways will have to be found to enhance uptake of the lipids by the brain. |
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