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A Quantitative Assay for Measuring of Bovine Immunodeficiency Virus Using a Luciferase-based Indicator Cell Line |
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| Authors: | Xue YAO Hong-yan GUO Chang LIU Xuan XU Jian-sen DU Hao-yue LIANG Yun-qi GENG Wen-tao QIAO Nankai University Tianjin China School of Medicine China |
| Institution: | Xue YAO1,Hong-yan GUO1,Chang LIU2,Xuan XU1,Jian-sen DU1,Hao-yue LIANG1,Yun-qi GENG1 , Wen-tao QIAO1 (1. Key Laboratory of Molecular Microbiology , Technology,Ministry of Education,Key Laboratory of Microbial Functional Genomics (Tianjin),Nankai University,Tianjin 300071,China,2. School of Medicine,China) |
| Abstract: | In order to quantitate the bovine immunodeficiency virus (BIV) infection in vitro, a BIV indicator cell line (BIVL) was established by transfecting baby hamster kidney cells with reporter plasmids containing the firefly luciferase gene driven by a BIV long terminal repeat promoter. The BIV activates promoter activity of the LTR to express luciferase upon infection. BIV infection could therefore by quantified by detection of luciferase activity. Compared to standard assays used to detect BIV infection, the B... |
| Keywords: | Bovine immunodeficiency virus (BIV) Bovine foamy virus (BFV) Luciferase Indicator cell line |
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