胶束电动毛细管色谱法测定红丝线提取物中紫蓝素的含量

建立了胶束电动毛细管电泳色谱法(MECC)测定红丝线提取物中紫蓝素的含量的方法。毛细管柱内径75μm,长50.2cm;运行电压25kV,检测波长585nm,温度25℃;缓冲液为25mmol/L,β-CD10mmol/L,硼酸盐-20%乙腈(pH值8.0);进样方式:压力进样,进样时间5s。结果表明紫蓝素在10～100μmol/L浓度范围内具有良好的线性关系(r=0.9995),回收率为95.3%～103.2%。该方法快速、简便、并且较为准确,适用于测定红丝线提取物中紫蓝素的含量。

Determination of zilanine contents in extracts of Peristrophe baphica by Micellar Eletrokinetic Capillary Chromatography(MECC)

The method of MECC was established to determine zilanine contents in extracts of P.baphica. The capillary column was 50.2 cm long and 75 μm in internal diameter;the operation voltage was 25 kV,testwavelength 585 nm,column temperature 25 ℃;the mobile buffer was 25 mmol/L boric acid-20% acetonitrile(pH 8.0)with 10 mmol/L β-CD;the sampling was conducted by pressure for 5 s. The results showed that the linear range of determination for zilanine was 10～100 μmol/L(r=0.9995). The average recovery rate was 95.3%-103.2%. The method was fast,simple,convenient,comparatively accurate,and could be used to determinate zilanine contents in extract of P.baphica.