Ultrastructural localization of chromogranin: a potential marker for the electron microscopical recognition of endocrine cell secretory granules |
| |
Authors: | I M Varndell R V Lloyd B S Wilson J M Polak |
| |
Institution: | (1) Department of Histochemistry, Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, W12 0HS London, UK;(2) Department of Pathology, University of Michigan Medical School, 48109 Ann Arbor, Michigan, USA |
| |
Abstract: | Summary Using a monoclonal antibody (LK2H10) directed against human chromogranin, we have been able to localize this soluble glycoprotein to the matrix of secretory granules from a wide variety of endocrine cells. In the gut, enterochromaffin, enteroglucagon, glucose-dependent insulinotropic peptide, gastrin, and neurotensin-containing cells exhibit chromogranin immunoreactivity. In our system, chromogranin-immunoreactive material was restricted to the halo of human pancreatic glucagon-containing secretory granules within A-cells. Chromogranin immunoreactivity was also localized to secretory granules in phaeochromocytomas, gastrinomas, medullary carcinomas of the thyroid and a carotid body tumour (chemodectoma). Chromogranin is proposed as a potential marker for the ultrastructural recognition of endocrine cell secretory granules. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|