Possible relationship between micronucleated and binucleated cells induced by cisplatin in cultured CHO cells |
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| Institution: | 1. Department of Anesthesiology and Intensive Care, Changhai Hospital, Second Military Medical University, Shanghai 200433, China;2. Shanghai Key Laboratory of Molecular Mycology, Department of Dermatology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China;3. Department of Anesthesiology, Fuzhou General Hospital of Nanjing Military Region, Fuzhou 350025, Fujian Province, China;1. Dept. of Psychology and Center \"Daniel Bovet\", Sapienza University, 00184 Rome, Italy;2. IRCSS Fondazione Santa Lucia, 00142 Rome, Italy;3. Behavioral Neuroscience PhD Programme, Sapienza University, 00184, Rome, Italy;4. Dept. of Applied and Biotechnological Clinical Sciences, University of L''Aquila, 67100 L''Aquila, Italy;5. Cell Biology and Neurobiology Institute, National Research Council, 00143 Rome, Italy;6. Institut Universitaire en Santé Mentale de Québec, Laval University, Quebec, Canada |
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| Abstract: | Chinese hamster ovary (CHO) cells were treated with a single dose (10 μg/ml) of cis-diammino-dichloroplatinum (II) (cisplatin) for 1 h and the effect of the drug on the kinetics of proliferation of the cultures was studied. It was found that the drug produces a delay in the proliferation rates of the treated cultures.The induction of micronuclei and binucleated cells (BC) at different times after treatment have also been studied, and the ability of these cells to undergo DNA synthesis (measured as the ability to incorporate 3H]thymidine) is shown.It was found that cisplatin induced a particular type of BC that contains one or more micronuclei rather than a pure population of BC. The results obtained show a possible relationship between micronuclei and BC. The possibility that some of the micronucleated cells evolve in subsequent cell divisions to BC with micronuclei is suggested. |
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