Comparison of the sensitivity of Salmonella typhimurium strains YG1024 and YG1012 for detecting the mutagenicity of aromatic amines and nitroarenes |
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| Affiliation: | 1. Laboratorio de Investigación en Patología Experimental, Hospital Infantil de México Federico Gómez, Mexico;2. Laboratorio de Investigación en Inmunología y Proteómica, Hospital Infantil de México Federico Gómez, Dr. Márquez 162, Colonia Doctores, 06720 Ciudad de México, Mexico;3. Unidad de Biología de Células Individuales (Biocelin), Laboratorio de Investigación en Patología Experimental, Hospital Infantil de México Federico Gómez, Mexico;4. Laboratorio de Especiación Química de Aerosoles Orgánicos Atmosféricos y Desarrollo de Tecnologías Verdes, Departamento de Ciencias Ambientales, Centro de Ciencias de la Atmósfera, Universidad Nacional Autónoma de México, 04510 Ciudad de México, Mexico;5. Unidad Universitaria de Investigación, División de Investigación, Facultad de Medicina de la Universidad Nacional Autónoma de México (UNAM), Mexico;6. Departamento de Fisiología y Neurociencias, Centro de Investigación y de Estudios Avanzados (CINVESTAV), Mexico |
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| Abstract: | Salmonella typhimurium YG1024 is a derivative of S. typhimurium TA98 with a high level of N-hydroxyarylamineO-acetyltransferase (OAT) activity. We have demonstrated that this strain is highly sensitive to the mutagenic actions of N-hydroxyarylamines derived from aromatic amines and nitroarenes. In this paper, we compared the sensitivities of YG1024 with those of S. typhimurium YG1012, which has about 4 times higher OAt activity than YG1024 but lacks plasmid pKM101. It turned out that YG1024 was more sensitive to the mutagenic actions of 1-aminonaphthalene, 1-nitropyrene, 1,8-dinitropyrene and 2-nitronaphthalene than YG1012 and showed comparable sensitivity to 2-hydroxyacetylaminofluorene, 2-aminoanthracene and 2-amino-6-methyldipyridol[1,2-α:3′,2′-d]imidazole (Glu-P-1) to YG1012. These results suggested that YG1024 is more suitable than YG1012 for the efficient detection of mutagenic aromatic amines and nitroarenes. |
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