Nitric oxide synthase induction in glial cells: Effect on neuronal survival |
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Institution: | 1. Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CONICET CCT La Plata, CIC.PBA, 47 y 116, La Plata, Buenos Aires, Argentina;2. Cátedra de Microbiología, Dpto. Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, 47 y 115, La Plata, Buenos Aires, Argentina;3. Instituto Multidisciplinario de Biología Celular (IMBICE); Universidad Nacional de La Plata, CONICET CCT La Plata, CIC; 526 y Camino Gral Belgrano, La Plata, Buenos Aires, Argentina;4. Universidad Autónoma de Barcelona (UAB), Barcelona, España;5. Área Bioquímica y Control de Alimentos, Dpto. Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata; 47 y 115, La Plata, Buenos Aires, Argentina |
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Abstract: | In primary rat cortical glial cell cultures lipopolysaccharide (LPS) induced a dose- and time-dependent increase of intracellular cyclic GMP concentration associated with a release of nitrite. The LPS-induced cyclic GMP and nitrite increase was enhanced by interferon-γ and was prevented by L-NG- nitroarginine, dexamethasone and cycloheximide. Thus indicates that LPS effect occured via the production of nitric oxide (NO) and involved new protein synthesis suggesting the induction of NO syntahse in these cells. Furthermore this induction was Ca2+-independent and was blocked by an inhibitor of the synthesis of tetrahydrobiopterin. The inducible NO synthase was also expressed by C6 glioma cells. In primary mixed cultures containing both neuronal and glial cells, the effects of LPS were less important than in primary glial cell cultures suggesting that glial cells rather than neurons expressed the inducible form of NO synthase. On the other hand no change on neuronal viability was observed after NO synthase induction by LPS in this culture type. This study indicates that glial cells are able to induce NO synthase without affecting neuronal survival. |
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