Activation of neutrophils NADPH oxidase by PMA: Cytosol activity is translocated in phorbol-primed neutrophils |
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| Institution: | 1. Department of Medicine, Fukuoka Dental College, Fukuoka 814-01, Japan;2. Cancer Center, Kyushu University School of Medicine, Fukuoka 812, Japan;3. First Department of Internal Medicine, Kyushu University School of Medicine, Fukuoka 812, Japan;1. Ruhr University Bochum, Institute of Biochemistry and Pathobiochemistry, Microbial Biochemistry, 44780 Bochum, Germany;2. Ruhr University Bochum, Medizinisches Proteom-Center, 44801 Bochum, Germany;1. Ruhr University Bochum, Institute of Biochemistry and Pathobiochemistry, Microbial Biochemistry, Bochum, Germany;2. Institute of Biochemistry, Centre for Human and Molecular Biology (ZHMB), Saarland University, 66123, Saarbrücken, Germany |
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| Abstract: | - 1.1. Translocation of cytosol activity in phorbol-primed neutrophils was studied.
- 2.2. Prior exposure of PMA or FMLP could potentiate the oxidative response by subsequent heterogeneous stimulus, FMLP or PMA.
- 3.3. In FMLP-primed neutrophils, the cytosol had almost the same activity as resting one and cytosol activity was not eluted from the membrane.
- 4.4. In PMA-primed neutrophils, however, the cytosol had less activity and cytosol activity was correspondingly eluted from the membrane.
- 5.5. These observations suggested that cytosol activity was translocated in PMA-primed cells.
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