Mitochondrial and peroxisomal fractions derived from human white adipocytes |
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| Institution: | 1. Department of Biochemistry, University of Cape Town, Rondebosch 7700, South Africa;2. Department of Microbiology and Biochemistry, University of the Orange Free State, PO Box 339, Bloemfontein 9300, South Africa;1. Department of Zoology and Environmental Science Gurukula Kangari Vishwavidyalaya, Haridwar, 249404, India;2. Institute of Environment and Development Studies, Bundelkhand University, Kanpur Road, Jhansi, 284128, India;3. Department of Botany, Dayalbagh Educational Institute, Agra, 282005, India;4. Centre for Environment Science and Climate Resilient Agriculture, Indian Agricultural Research Institute, New Delhi, 110012, India;5. Engineering Department, Razak Faculty of Technology and Informatics, Universiti Teknologi Malaysia, Jalan sultan Yahya Petra, 54100, Kuala Lumpur, Malaysia;1. Department of Landscape Plants, Sichuan Agricultural University, Chengdu 611130, China;2. College of Forest, Sichuan Agricultural University, Chengdu 611130, China;3. Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China;1. Department of Agricultural and Biological Engineering, The Pennsylvania State University, University Park, PA 16802, USA;2. Department of Agricultural and Biological Engineering, Gulf Coast Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, Wimauma, FL 33598, USA;3. Department of Surveying Engineering, The Pennsylvania State University, Dallas, PA 18612, USA;1. Department of Global and International Studies, University of Northern British Columbia, Prince George, British Columbia, Canada;2. Institute for the Oceans and Fisheries, University of British Columbia, Vancouver, British Columbia, Canada;3. Department of Geography, University of British Columbia, Vancouver, British Columbia, Canada;4. School of Public Policy and Global Affairs, University of British Columbia, Vancouver, British Columbia, Canada;5. Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada;6. Nippon Foundation Ocean Nexus Program, University of British Columbia, Vancouver, British Columbia, Canada;7. Department of Geography, Environment and Geomatics, University of Guelph, Guelph, Ontario, Canada;1. Austrian Centre of Industrial Biotechnology (ACIB GmbH), Muthgasse 18, Vienna, Austria;2. Institute of Microbiology and Microbial Biotechnology, BOKU-VIBT, University of Natural Resources and Life Sciences, Muthgasse 18, Vienna, Austria;3. Institute of Chemical, Environmental and Bioscience Engineering, TU Wien, Vienna, Austria;4. CD Laboratory for Biotechnology of Glycerol, Muthgasse 18, Vienna, Austria;5. Department of Applied Genetics and Cell Biology (DAGZ), University of Natural Resources and Life Sciences, Muthgasse 11, Vienna, Austria;1. Department of Ecology and Environmental Science, Umeå University, Sweden;2. Umeå Marine Sciences Centre, Umeå University, Hörnefors, Sweden |
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| Abstract: | - 1.1. A procedure is described for the separation of intact peroxisomes from human white adipocytes using a linear metrizamide gradient (20–50% w/v).
- 2.2. Peroxisomes were found in the high density region of the gradient in an intact form.
- 3.3. Mitochondria were distributed in the high density and low density regions of the gradient.
- 4.4. Lysosomes separated well from the peroxisomes, occurring only in the low density region of the gradient.
- 5.5. Low levels of glyoxylate cycle enzyme activities (isocitrate lyase and malate synthase) were detected within the light and heavy mitochondrial pellet fractions.
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