Degradation of bone matrix proteins by osteoclast cathepsins |
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| Institution: | 1. Department of Nutrition and Food Safety, School of Public Health, Nanjing Medical University, Nanjing 211166, China;2. The Key Laboratory of Modern Toxicology, Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, China;1. Department of Animal Science, Aarhus University, Tjele, Denmark;2. The Roslin Institute and Royal (Dick) School of Veterinary Sciences, University of Edinburgh, Easter Bush, Midlothian, UK;3. Department of Virology, Immunobiology and Parasitology, National Veterinary Institute, Uppsala, Sweden;1. Key Laboratory of Meteorological Disaster of Ministry of Education, Collaborative Innovation Centre on Forecast and Evaluation of Meteorological Disasters, School of Atmospheric Physics, Nanjing University of Information Science and Technology, Nanjing, 210044, China;2. State Key Laboratory of Atmospheric Boundary Layer Physics and Atmospheric Chemistry, Institute of Atmospheric Physics, Chinese Academy of Sciences, Beijing, 100029, China;3. Department of Industrial and Systems Engineering, The Hong Kong Polytechnic University, Hong Kong, SAR, China;4. New South Wales Office of Environment and Heritage, Sydney, Australia;5. Department of Environment and Geography, Macquarie University, Sydney, Australia;6. Shanghai General Hospital, Shanghai, China;7. Jiangsu Key Laboratory of Agricultural Meteorology, School of Applied Meteorology, Nanjing University of Information Science and Technology, Nanjing 210044, China;1. Department of Pharmacology & Therapeutics, Roswell Park Comprehensive Cancer Center, Buffalo, NY, 14263, USA;2. Key Lab of Agricultural Animal Genetics, Breeding & Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China;3. Cancer Stem Cell Institute, Research Center for Translational Medicine, East Hospital, Tongji University School of Medicine, Shanghai, 200120, China |
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| Abstract: | - 1.1. The degradation of the bone matrix proteins osteocalcin, osteonectin and α2HS-glycoprotein by human cathepsins B and L and human osteoclastoma cathepsins has been investigated.
- 2.2. Intermediate degradation products (Mr > 12kDa) were not observed during the digestion of α2HS-glycoprotein and osteonectin by cathepsins B and L although they were observed with some of the osteoclastoma cathepsins. Most of the osteoclastoma cathepsins were capable of degrading these two proteins to small peptides at comparable rates.
- 3.3. Each cathepsin produced a different pattern of osteocalcin degradation products.
- 4.4. The extensive range of non-collagenous proteins in bone matrix may necessitate the production by osteoclasts of cathepsins with different specificities during bone resorption.
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