Distribution of beta-adrenergic binding in fractionated porcine adipocytes |
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| Institution: | 1. Department of Interdisciplinary Studies of Culture, Norwegian University of Science and Technology (NTNU), Postal address: Department of Interdisciplinary Studies of Culture, NTNU. NO-7491, Trondheim, Norway;2. Department of Neuromedicine and Movement Science, Norwegian University of Science and Technology (NTNU). Postal address: NTNU, Faculty of Medicine and Health Sciences, N-7491 Trondheim, Norway;3. Department of Architecture and Planning, Norwegian University of Science and Technology (NTNU), Postal address: Department of Architecture and Planning, NTNU, 7491, Trondheim, Norway.;1. Translational and Evolutionary Genomics Lab, School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India;2. Department of Biotechnology, Delhi Technological University, Delhi 110042, India;3. Department of Plant Science, School of Biological Sciences, Central University of Kerala, Kararagod 671316, India;4. Department of Bioengineering and Technology, Institute of Science and Technology,?Gauhati University,?Gopinath Bordoloi Nagar, Guwahati 781014, India;5. School of Computational and Integrative Sciences,?Jawaharlal Nehru University, New Delhi 110067, India;1. University College London, Interaction Centre, Department of Computer Science, Faculty of Engineering, 66-72 Gower Street, London, WC1E 6BT, UK;2. Imperial College London, Grantham Institute, South Kensington Campus, London, SW7 2AZ, UK;3. University College London, The Bartlett School of Environment, Energy and Resources, Energy Institute, Faculty of the Built Environment, 14 Upper Woburn Place, London, WC1H 0NN, UK |
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| Abstract: | - 1.1. The subcellular distribution of the porcine adipocyte beta-adrenergic receptor was studied in fractionated adipocytes.
- 2.2. The 30,000 g pellet obtained from hypotonically lysed cells contained membrane vesicles and mitochondria; it yielded approx 200–300 fmol dihydroalprenolol-bound receptors/mg protein.
- 3.3. Activity was increased to about 1000 fmol/mg protein after isolation of a plasma membrane fraction on a Percoll gradient.
- 4.4. The 5'-nucleotidase, succinate dehydrogenase and lactate dehydrogenase activities were usually enriched in compartments different from the ligand-binding activity.
- 5.5. Activity of porcine adipocyte 5'-nucleotidase, a purported plasma membrane marker enzyme, was not distributed in the same manner as the beta-adrenergic receptor.
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