Priming with murine recombinant interleukin-5 resulted in the augmentation of PAF-induced airway hyperresponsiveness to histamine in guinea pigs |
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| Institution: | 1. Department of Pharmacology, Gifu Pharmaceutical University, 5-6-1, Mitahorahigashi, Gifu, Japan;1. Suntory Institute for Biochemical Research, Mishima, Osaka, Japan;1. Faculty of Medicine, Kermanshah University of Medical Science, Kermanshah, Iran;2. Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran;3. Universal Scientific Education and Research Network (USERN) Office, Kermanshah University of Medical Sciences, Kermanshah, Iran;4. Clinical Research Development Center, Imam Khomeini and Mohammad Kermanshahi and Farabi Hospitals, Kermanshah University of Medical Sciences, Kermanshah, Iran;5. Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran;6. Department of Tissue Engineering, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran;1. Chronic Airways Diseases Laboratory, Department of Respiratory and Critical Care Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China;2. Chronic Airways Diseases Laboratory, Department of Respiratory and Critical Care Medicine, The Fifth Affiliated Hospital of Southern Medical University, Guangzhou, China;3. School of Public Health, Southern Medical University, Guangzhou, China;4. Department of Endocrinology, Nanfang Hospital, Southern Medical University, Guangzhou, China;1. State Key Laboratory of Resource insects, Southwest University, Chongqing 400716, China;2. Jinfeng Laboratory, Chongqing 401329, China;3. Chongqing engineering and Technology Research Center for Silk Biomaterials and Regenerative medicine, Chongqing 400716, China |
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| Abstract: | The effects of pretreatment with murine recombinant interleukin 5 (mrIL-5) on platelet activating factor (PAF)-induced bronchoconstriction and airway hyperreactivity were investigated in guinea pigs. The intratracheal administration of mr IL-5 (2.5–10 μg) augmented platelet activating factor (PAF; 50 ng/kg)-induced bronchoconstriction in guinea pigs. When IL-5 (2.5 μg) was injected intratracheally, PAF (25 ng/kg)-induced bronchoconstriction was not affected, but PAF-induced airway hyperresponsiveness to histamine was exacerbated. Airway inflammation, in terms of increased capillary permeability and the accumulation of leukocytes in bronchoalveolar lavage fluid, was not produced by pretreatment with PAF (25 ng/kg), mrIL-5 (2.5 μg), or by a combination of these agents. This mrIL-5-induced augmentation of airway hyperreactivity by PAF was clearly inhibited by the phosphodiesterase-type III inhibitors, SDZ-MKS-492 and AH 21–132, but not by aminophylline. |
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