Localization of the glucocorticoid receptor in rat liver cells: Evidence for plasma membrane bound receptor |
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| Institution: | 1. Institute of Biological Research and Biotechnology, National Hellenic Research Foundation, 48, Vassileos Constantinou Avenue, Athens 11635, Greece [Tel. 721-0274];2. German Cancer Research Center, Heidelberg, Germany;1. Department of Orthopedic Surgery, Korea University Guro Hospital, College of Medicine, Korea University, Seoul, Korea;2. Department of Orthopedic Surgery, Inje University Sanggye Paik Hospital, College of Medicine, Inje University, Seoul, Korea;1. Hotchkiss Brain Institute, University of Calgary, Calgary, AB, Canada;2. Mathison Centre for Mental Health Research and Education, University of Calgary, Calgary, AB, Canada;3. Departments of Cell Biology and Anatomy and Psychiatry, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada;1. Assistance Publique Hôpitaux de Paris, Pitié-Salpêtrière Hospital, Department of Metabolic Biochemistry, 75013 Paris, France;2. Hematology Division, Brigham and Women''s Hospital, Dana Farber Cancer Institute, Boston, MA, USA;3. Harvard Medical School, Boston, MA, USA;4. Assistance Publique Hôpitaux de Paris, Pitié-Salpêtrière Hospital, Intensive Care Unit, 75013 Paris, France;5. Assistance Publique Hôpitaux de Paris, Pitié-Salpêtrière Hospital, Department of Hematology, 75013 Paris, France;6. Assistance Publique Hôpitaux de Paris, Saint-Louis Hospital, Department of Internal Medicine, Autoimmune and Vascular Disease Unit, 75010 Paris, France;7. McGill University, Department of Medicine, Montreal, Québec, Canada;8. Paris University, UTCBS, INSERM U1267, CNRS UMR 8258, 75006 Paris, France;9. Paris University, EA 3518, University Institute of Hematology, 75010 Paris, France;10. Sorbonne University, INSERM UMRS_1166, Institute of Cardiometabolism And Nutrition, 75013 Paris, France;1. The State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei Province, China;2. College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei Province 430070, China;3. Hubei International Scientific and Technological Cooperation Base of Veterinary Epidemiology, Key Laboratory of Preventive Veterinary Medicine in Hubei Province, Huazhong Agricultural University, Wuhan, Hubei Province 430070, China;4. School of Veterinary Medicine, Murdoch University, 6150, Australia |
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| Abstract: | - 1.1. The cytoplasmic glucocorticoid receptor of rat liver cells is in part recovered in the plasma membrane fraction.
- 2.2. After in vivo administration of 3H]dexamethasone, 0.35% of the radioactivity recovered is bound on plasma membranes.
- 3.3. Dexamethasone also binds in vitro specifically to plasma membranes. Expressed as fmol/mg protein, binding of dexamethasone to plasma membranes is comparable to binding to the soluble cytoplasmic fraction (cytosol).
- 4.4. Using polyclonal antibody to the glucocorticoid receptor and the indirect immunofluorescence technic, an intense decoration of the plasma membranes is observed, denoting a high concentration of glucocorticoid receptor on plasma membranes.
- 5.5. The localization of the receptor on plasma membranes could be of potential importance for its interaction with agents (mitogens, growth factors) initially acting on the cell membrane, regulating subsequent cell proliferation and growth at the level of the cell nucleus.
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