Melatonin-like Immunoreactivity in the Presence of Different Chemicals as Determined by the Radioimmunoassay |
| |
| Institution: | 1. Department of Chemical Engineering, Imperial College London, London SW7 2AZ, United Kingdom;2. Bristol-Myers Squibb, Reeds Lane, Moreton, Wirral, Merseyside, CH46 1QW, United Kingdom;1. Analytical Chemistry Department, Faculty of Pharmacy, October 6 University, 6 October City, Giza, Egypt;2. Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, El-Kasr El-Aini Street, ET-11562, Cairo, Egypt;3. Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University (Girls), Cairo, Egypt;4. Analytical Chemistry Department, Faculty of Pharmacy, Egyptian Russian University in Egypt, Badr City, Cairo, Egypt;1. Department of Chemical Engineering, Imperial College London, London SW7 2AZ, United Kingdom;2. Analytical & Quality Evaluation Research Laboratories, Daiichi Sankyo Co. Ltd., 1-12-1, Shinomiya, Hiratsuka-shi, Kanagawa, 254-0014, Japan;1. Division of Food, Medicines and Consumer Safety, Section Medicinal Products, Scientific Institute of Public Health (WIV-ISP), J. Wytsmanstraat 14, B-1050 Brussels, Belgium;2. Research Group NatuRA (Natural Products and Food – Research and Analysis), Department of Pharmaceutical Sciences, University of Antwerp, Universiteitsplein 1, B-2610 Wilrijk, Belgium;1. Department of Chemical Engineering, Imperial College London, London SW7 2AZ, United Kingdom;2. Bristol-Myers Squibb, Reeds Lane, Moreton, Wirral, Merseyside CH46 1QW, United Kingdom |
| |
| Abstract: | To determine the nature of the molecule(s) that is responsible for the melatonin like immunoreactivity (MLI), we measured the effect of pretreatment of plasma samples with detergents, reducing agent, and proteinase K. Nonidet P-40 Triton X-100, and ethylacetate extraction had no effect, while sodium deoxycholate, sodium dodecyl sulfate, β-mercaptoethanol, ether extraction, proteinase K, and temperature increased the MLI. Since the radioimmunoassay (RIA) was sensitive to proteinase K, ionic detergents, and a reducing compound, we hypothesize that a proteinaceous molecule might be responsible for this MLI. We compared our column procedure for RIA of plasma melatonin (1) with procedures involving extraction with either ethylacetate or ether. In our hands preextraction of samples with organic solvents caused a loss of immunoreactivity. We also found that passing samples through the column is more efficient in eliminating interference in the melatonin assay than extracting samples either with ethylacetate or ether. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
