1H-NMR characterization of the human myocardium myoglobin and erythrocyte hemoglobin signais |
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| Institution: | 1. Department of Pediatrics and Medicine, Neuromuscular Clinic, McMaster University, Hamilton, Ontario L8N 3Z5, Canada;2. Department of Pediatrics, Division of Medical Genetics/Duke University Medical Center, Durham, NC, USA;3. Department of Bioenergetics and Exercise Physiology, Medical University of Gdansk, Poland;1. The Russell H. Morgan Department of Radiology, The Johns Hopkins University School of Medicine, 991 N. Broadway Baltimore, MD 21205, USA;2. The National High Magnetic Field Laboratory, CIMAR, Florida State University, Tallahassee, FL, USA;3. Department of Chemical and Biomedical Engineering, FAMU-FSU College of Engineering, Tallahassee, FL, USA;4. F.M. Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Institute, 707 N. Broadway Ave., Baltimore, MD 21205, USA;1. Department of Biomedical Engineering, Zhejiang University, Hangzhou, China;2. School of Information Technology and Electrical engineering, The University of Queensland, Australia;3. Division of Superconducting Magnet Science and Technology, Institute of Electrical Engineering, Chinese Academy of Sciences, China;1. Cell and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA |
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| Abstract: | The 1H-NMR signal of deoxy Mb provides a unique opportunity to measure tissue oxygenation in vivo. To utilize the technique for human application, however, requires a specific spectral characterization of both human Mb and erythrocyte Hb. We report that the proximal histidyl-NH signal of human deoxy Mb resonates at 80.3 ppm at 25°C and maintains a 3.9 ppm separation with the corresponding Hb A signal throughout the physioiogical temperature range. In the particular case of the human thenar muscle, the deoxy Mb signal is clearly detectable without any interference from Hb. |
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