Monoclonal antibodies prepared against heparin lyase I and their reactivity toward heparin lyase I,II and III |
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| Institution: | 3. Division of Immunochemistry/Bioanalytical Chemistry, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Parkallee 4a, 23845 Borstel, Germany,;4. Infection Biology, Biozentrum, University of Basel, Klingelbergstrasse 50/70, CH-4056 Basel, Switzerland, and;5. Department of Biology, University of Namur, B5000 Namur, Belgium |
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| Abstract: | - 1.1. Six different monoclonal IgG mouse antibodies to heparin lyase I from Flavobacterium heparinum were prepared.
- 2.2. The monoclonal antibodies were used to detect heparin lyases I, II and III by dot-blotting immunoassay and by Western blotting.
- 3.3. Individual antibodies showed different reactivity toward the three heparin lyases.
- 4.4. The reactivity of two of the monoclonal antibodies was destroyed by exposing heparin lyases to sodium dodecyl sulfate.
- 5.5. The antibodies can be used to rapidly distinguish between the three heparin lyases.
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