Subcellular and developmental studies of the tyrosyl protein sulfotransferase in rat brain |
| |
| Institution: | 1. Discipline of Chemistry, University of Newcastle, Callaghan, NSW 2308, Australia; University of New South Wales, School of Material Science and Engineering, NSW 2052, Australia;2. School of Chemical Engineering University of Newcastle, Callaghan, NSW 2308 Australia;3. School of Materials Science and Engineering, University of NSW, Kensington, NSW 2052, Australia;4. Electron Microscopy Centre, Australian Institute for Advanced Materials, Innovation Campus, University of Wollongong, Squires Way, North Wollongong, NSW 2517, Australia;5. Department of Soil Science and Plant Nutrition, Working Group Climate Change Research for Special Crops, University Geisenheim, Von-Lade Str. 1, D-65366 Geisenheim, Germany;6. School of GeoSciences, University of Edinburgh, Crew Building, Alexander Crum Brown Road, EH9 3BZ, Edinburgh, UK;7. Dipartimento di Scienze Agrarie, Alimentari e Forestali, Università degli Studi di Palermo, viale delle Scienze ed. 4, 90128 Palermo, Italy;8. Ithaka Institute for Carbon Strategies, Ancienne Eglise 9, 1974 Arbaz, Switzerland;9. Geomicrobiology, Center for Applied Geoscience, University of Tuebingen, Sigwartstrasse 10, 72076 Tuebingen, Germany;10. Solid State and Elemental Analysis Unit, Mark Wainwright Analytical Centre, University of New South Wales, Kensington, NSW 2052, Australia;11. United States Department of Agriculture, Agricultural Research Service, Soil and Water Management Unit, 1991 Upper Buford Circle, St. Paul, MN, USA;12. Institute of Soil, Water and Environmental Sciences, The Volcani Center, Agricultural Research Organization, P.O.B. 15159, Rishon LeTzion 7528809, Israel;1. Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, SP, Brazil;2. Faculty of Medicine, University Center of Espírito Santo, Colatina, ES, Brazil;1. Department of Gynecologic Oncology, Moffitt Cancer Center, Tampa, FL, United States of America;2. Department of Pathology, University of Washington, Seattle, WA, United States of America;3. Department of Cancer Epidemiology, Moffitt Cancer Center, Tampa, FL, United States of America;4. Department of Pathology, Division of Women''s and Perinatal Pathology, Brigham and Women''s Hospital and Harvard Medical School, Boston, MA, United States of America;5. Department of Epidemiology, Harvard T.H. Chan School of Public Health, Boston, MA, United States of America |
| |
| Abstract: | - 1.1. Tyrosyl protein sulfotransferase (TPS) activity in the newborn and mature rat brain was studied using the cholecystokinin derivative terbutyloxycarbonyl-Asp-Tyr-Met-Gly-Trp-Met-Asp-PheNH2, BocCCK-8(ns), as the peptide substrate.
- 2.2. TPS activity was enriched 4 times in the microsomal and synaptic vesicular enriched fractions of rat cerebral cortex.
- 3.3. CCK-8 content, in the subcellular fractions and the peptide sulfation activity distribution was in accord with the hypothesis that tyrosyl protein sulfotransferase plays a key role in the maturation process of bioactive CCK.
- 4.4. TPS activity measured in membranes from newborn brain was 2.5 times higher than the activity observed in the mature brain membranes with a Vmax = 0.83 ± 0.05 and 0.31 ± 0.02 respectively. The apparent KM for the sulfate donor, 3'-phosphoadenosine 5'-phosphosulfate (PAPS), was similar, 94 ± 4 nM and 90 ± 6 nM and the kM for the peptide substrate, BocCCK-8(ns), was 234 ± 16 μM and 160 ± 12 μM in the newborn and adult brain membranes respectively.
- 5.5. TPS activity reached normal mature values within 20 days of age.
- 6.6. These data support the idea that tyrosyl protein sulfation is an important process in the secretion mechanism and in the CCK maturation.
|
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
