Role of glutamate dehydrogenase reaction in the control of citrate pool in yeast |
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| Institution: | 1. Universidade de Ribeirão Preto (UNAERP), São Paulo, Brazil 14096-900;2. Programa de Pós-Graduação em Nutrição e Metabolismo, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil 14049-900;3. Curso de Nutrição e Metabolismo, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil. 14049-900;4. Programa de Pós-Graduação em Saúde Pública, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil 14049-900;5. Cancer Prevention and Control Program and Department of Epidemiology and Biostatistics, University of South Carolina, Columbia, SC 29208, USA;6. Departamento de Medicina Social, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo. , São Paulo, Brazil 14049-900;1. Research Planning, Inc. (RPI), 247 E. 7th Avenue, Suite 200, Tallahassee, FL 32303, USA;2. U.S. Fish and Wildlife Service, 61389 Highway 434, Lacombe, LA 70445, USA;3. Louisiana Department of Environmental Quality, 201 Evans Road, Suite 420, New Orleans, LA, 70123, USA;4. Texas Petroleum Investment Company, 207 Town Center Parkway, Suite 150, Lafayette, LA 70506, USA;5. Louisiana State University (LSU), Department of Environmental Sciences, Baton Rouge, LA 70803, USA;6. NOAA Emergency Response Division, Office of Response and Restoration, 500 Poydras Street, Suite 1213, New Orleans, LA 70130, USA |
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| Abstract: | - 1.1. Role of NADP-glutamate dehydrogenase in the depletion of citrate was analyzed using permeabilized yeast cells.
- 2.2. Citrate was converted to 2-oxoglutarate, which was then metabolized to glutamate by NADP-glutamate dehydrogenase in the presence of ammonium ion.
- 3.3. Formation of 2-oxoglutarate plus glutamate was in good agreement with the concentration of citrate decreased. Glutamate formation can be a good indicator of the depletion of citrate, because 70% of the citrate decreased was converted to glutamate.
- 4.4. Glycolytic activity was closely correlated with the decrease in citrate under the in situ conditions.
- 5.5. NADP-glutamate dehydrogenase increased in anaerobically grown yeast cells.
- 6.6. An effective depletion of citrate by increased synthesis of NADP-glutamate dehydrogenase can explain the lowered mechanism of citrate causing glycolytic stimulation under the anaerobic growth conditions of yeast.
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