Lactoferrin binding to human platelets |
| |
| Institution: | 1. Department of Bioinformatics, Rajendra Memorial Research Institute of Medical Sciences, Patna 800007, India;2. Department of Biotechnology and Department of Pharmacoinformatics, National Institutes of Pharmaceutical Education and Research, Hajipur 844102, India;3. Institute for Protein Research, Osaka University, Suita 5650871, Japan;4. Division of Immunology, Rajendra Memorial Research Institute of Medical Sciences, Patna 800007, India;5. Dept. of Molecular Parasitology, Rajendra Memorial Research Institute of Medical Sciences, Patna 800007, India |
| |
| Abstract: | - 1.1. Platelets bind specifically lactoferrin.
- 2.2. The lactoferrin binding to the platelets depends on the concentration of labelled lactoferrin, the number of platelets, the time of incubation and pH.
- 3.3. The binding was characterized by two types of binding site: one with high affinity and low capacity, and another with low affinity and high capacity (respectively kaff 1 = 13.6 × 1091/mol and about 40 binding sites, and Kaff 2 = 1.23 × 1091/mol and about 135 binding sites per platelet).
- 4.4. Both human transferrin and bovine lactoferrin compete with human lactoferrin for the receptors.
- 5.5. The presence of lactoferrin receptors on the platelet membrane surface is connected most probably with the effect(s) on the cell function(s) of these cells.
|
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
