Phosphatidylethanolamine: Ceramide-ethanolaminephosphotransferase activity in synaptic plasma membrane vesicles. Influence of some cations and phospholipid environment on transferase activity. Further proof of its location |
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| Institution: | 1. Department of Psychological and Brain Sciences, Indiana University, Bloomington, IN, USA;2. Gill Center for Biomolecular Neuroscience, Indiana University, Bloomington, IN, USA;3. Université Paris Diderot, Sorbonne Paris Cité, Unité de Biologie Fonctionnelle et Adaptative, CNRS UMR 8251, F-75205 Paris, France;1. Division of Bacterial Diseases, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences Harbin, China;2. School of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou, China;3. Key Laboratory of Saline-Alkali Vegetation Ecology Restoration, Northeast Forestry University, Ministry of Education Harbin, China;4. College of Life Science, Northeast Forestry University Harbin, China;5. Department of Pharmaceutical Biology, Institute of Pharmaceutical and Biomedical Sciences, Johannes Gutenberg University, Mainz 55128, Germany;6. Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety and Beijing Laboratory for Food Quality and Safety, China Agricultural University, Beijing, China |
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| Abstract: | - 1.1. Synaptic plasma membrane vesicles (SPMV) from rat brain synthesized ceramide-phosphoethanolamine (SpE), an analogue of sphingomyelin (SpC) from phosphatidylethanolamine (PE) and ceramide.
- 2.2. This reaction was catalyzed by PE: ceramide-phosphotransferase.
- 3.3. The presence of PC did not modify the SpE synthesis and PI and PS at twice PE concentration seemed to be activators; only PG was an inhibitor at all concentrations.
- 4.4. Some cations (Mg2+, Mn2+) were without effect, while Ca2+ increased transferase activity, so was interesting to study.
- 5.5. Transferase was compared with sialidase (external enzyme).
- 6.6. Kinetics other than those already performed by us were undertaken in order to confirm its location.
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