Inhibition kinetics of brain butyrylcholinesterase by Cd2+ and Zn2+, Ca2+ or Mg2+ reactivates the inhibited enzyme |
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| Institution: | 1. UC Santa Cruz, CA, USA;2. University of Rhode Island, RI, USA;3. Dalhousie University, Halifax, NS, Canada;4. UC Santa Cruz, CA, USA;1. Department of Biochemistry, University of Maiduguri, P.M.B. 1069 Maiduguri, Nigeria;2. Department of Biochemistry, Federal University, Dutse, P. M. B. 7156 Dutse, Nigeria;3. Department of Biochemistry, Bayero University, Kano, P.M.B. 30ll Kano, Nigeria;4. Department of Biochemistry, Ahmadu Bello University, Zaria, P.M.B. 1045 Zaria, Nigeria;1. Eppley Institute, University of Nebraska Medical Center, Omaha, NE 68198-5950, USA;2. Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Rochester, MN 55905, USA;1. Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, School of Chemical & Materials Engineering, Jiangnan University, Wuxi 214122, PR China;2. Zanyu Technology Group Co. Ltd., Hangzhou 310009, PR China;1. Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, El-Kasr El-Aini Street, ET-11562 Cairo, Egypt;2. Analytical Chemistry Department, Faculty of Pharmacy, October 6 University, 6 October City, Giza, Egypt;3. Analytical Chemistry Department, Faculty of Pharmacy, Modern University for Technology and Information, Cairo, Egypt;1. Department of Medical Biochemistry, Faculty of Medicine, Hacettepe University, 06100, Ankara, Turkey;2. Department of Medical Biochemistry, Faculty of Medicine, Mugla Sitki Kocman University, 48000, Mugla, Turkey;3. Department of Medical Biochemistry, Faculty of Medicine, Yuksek Ihtisas University, 06520, Ankara, Turkey;4. Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Hacettepe University, 06100, Ankara, Turkey |
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| Abstract: | - 1.1. The inhibition kinetics of sheep brain butyrylcholinesterase (BChE) (acylcholine acylhydrolase, EC 3.1.1.8) by Cd2+ and Zn2+ has been studied.
- 2.2. Ks has been determined as 0.14mM. Cd2+ and Zn2+ were the hyperbolic mixed-type inhibitors of BChE. Ca2+ and Mg2+ had no effect on the enzyme activity in the experimental conditions.
- 3.3. But when the enzyme was inhibited by 0.1 mM Cd2+ or Zn2+, Ca2+ and Mg2+ reactivated the inhibited form of BChE.
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