Demonstration of highly specific and sensitive antibodies to a naturally occurring tetrahydroisoquinoline alkaloid,salsolidine |
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| Institution: | 1. Dokuz Eylül University, Institute of Marine Science and Technology, Haydar Aliyev Bul. No: 100, 35340 ?nciralt?-?zmir, Turkey;2. MTA Department of Geological Research, Üniversiteler Mahallesi, Dumlup?nar Bulvar?, TR-06800 Çankaya-Ankara, Turkey;3. Dokuz Eylül University, Department of Geology Engineering, Kaynaklar Kampus, T?naztepe, 35160 Buca-?zmir, Turkey;4. MTA Natural History Museum, Üniversiteler Mahallesi, Dumlup?nar Bulvar?, TR-06800 Çankaya-Ankara, Turkey;5. Muséum national d''histoire naturelle, UMR 7207 CNRS, MNHN, Univ Paris, 8 rue Buffon, 75005 Paris, France;6. Hacettepe University, Department of Geology Engineering, 06800 Beytepe-Ankara, Turkey |
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| Abstract: | - 1.1. We report for the first time on the production and characterization of antibodies against a naturally occurring tetrahydroisoquinoline, namely salsolidine (6,7-dimethoxy-1-methyl-1,2,3,4-tetrahydroisoquinoline).
- 2.2. Immunogen synthesis was carried out by coupling the hapten salsolidine to bovine serum albumin (BSA) as carrier protein on the basis of reductive amination.
- 3.3. By immunization of rabbits with salsolidine-BSA conjugate antisalsolidine antibodies were produced.
- 4.4. At a final dilution of 1:1700 the highest-litre antiserum bound 35% of 0.21 pmol 3H] salsolidine. This antiserum was used to develop a radioimmunoassay for salsolidine.
- 5.5. Cross-reactivity studies revealed a high specificity of the antiserum to the hapten.
- 6.6. The antibodies had a high affinity to salsolidine (Ka = 1.5 × 109 M?1).
- 7.7. Standard curves covered a measuring range of 0.5–70 pmol/tube and the detection limit was found to be 0.27 pmol/tube.
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