Solubilization and characterization of functionally coupled Escherichia coli heat-stable toxin receptors and particulate guanylate cyclase associated with the cytoskeleton compartment of intestinal membranes |
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| Institution: | 2. Departments of Medicine and Medical Microbiology and Immunology, Stanford University, Palo Alto, CA 94305, U.S.A.;1. KU Leuven, Department of Mechanical Engineering and Flanders Make@KU Leuven-MaPS, 3001 Leuven, Belgium;2. KU Leuven, Department of Materials Engineering, 3001 Leuven, Belgium;3. University of Siegen, Department of Chemistry and Biology, 57076 Siegen, Germany;1. Sultan Qaboos University, Sultanate of Oman;2. Sultan Qaboos Comprehensive Cancer Care and Research Center, Sultanate of Oman;3. Royal Hospital, Sultanate of Oman;4. Oman Medical Specialty Board Council, Sultanate of Oman;5. Sultan Qaboos University Hospital, Sultanate of Oman;6. Medical Oncology Department, Alzahra Hospital Dubai, Dubai, United Arab Emirates;7. Department of Medicine, University of Sharjah, Sharjah, United Arab Emirates;8. Emirates Oncology Society, Dubai, United Arab Emirates |
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| Abstract: | - 1.1. Particulate guanylate cyclase and receptors for E. coli heat-stable enterotoxin were solubilized from the rat intestinal cytoskeletal compartment using Lubrol-PX and KC1.
- 2.2. Thirty to forty percent of the ST receptor and guanylate cyclase activities were extracted from the lipid layer with Lubrol-PX alone.
- 3.2. Seventy percent of the remaining activities were solubilized from the cytoskeleton with Lubrol-PX and KCl.
- 4.3. Guanylate cyclase solubilized from either compartment exhibited similar reaction kinetics.
- 5.4. Both high- and low-affinity classes of ST receptors were solubilized from the lipid and cytoskeleton compartments.
- 6.5. In the presence of ATPγS, ST selectively activated the guanylate cyclase solubilized from the cytoskeleton compared to that solubilized from the lipid bilayer.
- 7.6. Crosslinking experiments demonstrated a preferential solubilization of the 130 kDa receptor subunit from the cytoskeleton and the 56 kDa subunit from the lipid bilayer.
- 8.7. Development of a procedure to solubilize ST receptors and guanylate cyclase from the intestinal membrane cytoskeleton will permit purification and further detailed studies of the coupling of these activities.
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