Prolactin receptors in rat cholangiocytes: Regulation of level and isoform ratio is sex independent |
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Authors: | R L Bogorad T Y Ostroukhova A N Orlova P M Rubtsov O V Smirnova |
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Institution: | (1) Faculty of Biology, Lomonosov Moscow State University, 119992 Moscow, Russia;(2) Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, ul. Vavilova 32, 119991 Moscow, Russia |
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Abstract: | The presence of prolactin receptor and peculiarities of its isoform expression in bile duct cells (cholangiocytes) differentially
isolated from rat liver under different conditions were investigated in the present study. Normal cholangiocytes express prolactin
receptor at relatively low level comparable to those of some prolactin-dependent tissues. Long receptor isoform is predominant
in cholangiocytes but not in hepatocytes. The prolactin receptor level increases significantly under obstructive cholestasis
due to evaluation of long and appearance of short isoforms. In rat cholangiocytes, unlike other tissues, the main positive
regulators of prolactin receptor expression are cholestasis-induced factors instead of sex hormone and prolactin levels. Long
isoform is predominant and induced primarily by cholestasis-induced factors.
Published in Russian in Biokhimiya, 2006, Vol. 71, No. 2, pp. 229–236. |
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Keywords: | cholestasis bile duct ligation RT-PCR cholangiocyte prolactin receptor receptor isoforms |
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