Identification of mitochondrial plasmid-like DNAs in Picea abies (L.) Karst. |
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| Authors: | A Chandelier P du Jardin C Avril M Pâques |
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| Institution: | Département de Biochime et Biologie Appliquées, Unité de Biologie Végétale, Faculté Universitaire des Sciences Agronomiques de Gembloux, Avenue Maréchal Juin, Batiment 48, B-5030 Gembloux, Belgium, BE
AFOCEL, Station de Biotechnologies, Domaine de l'Etan?on, F-77370 Nangis, France, FR
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| Abstract: | The Norway spruce (Picea abies (L.) Karst.) mitochondrial DNA has been extracted from embryonal suspensor masses. In addition to a master chromosome, a
family of plasmid-like DNAs were identified. These latter shared cross homologies but had no evident sequence homology with
the master chromosome. The occurrence of mitochondrial plasmid-like DNAs was investigated in trees from different provenances.
A vast majority of trees displayed extrachromosomal DNA elements of variable stoechiometry. For some trees, the sequences
homologous to the extrachromosomal DNA elements were found associated with high molecular weight DNA.
Received: 9 July 1997 / Revision received: 29 January 1998 / Accepted: 29 November 1998 |
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| Keywords: | Mitochondrial DNA Mitochondrial plasmid-like DNA Picea |
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