斑茅割手密复合体(GXAS07-6-1)及其与甘蔗F_1的GISH分析

斑茅割手密复合体(GXAS07-6-1)是广西蔗茅属斑茅和广西甘蔗属割手密的属间杂种,聚集了双亲的优点。本研究利用基于Alu-like的PCR鉴定方法对GXAS07-6-1及甘蔗与GXAS07-6-1的3份F_1材料进行真实性鉴定,基于基因组原位杂交技术(GISH)对父本GXAS07-6-1及其3份F_1染色体组成及核型进行分析。研究结果表明:3份F_1材料为GXAS07-6-1的真杂种;父本GXAS07-6-1的染色体众数为62条,其中30条来自蔗茅属斑茅,32条来自甘蔗属割手密,核型分类属于1B,其染色体按"n+n"方式传递;GXASF_108-2-17、GXASF_108-2-22、GXASF_108-2-32的染色体数目为78~80条,其中69~71条来自甘蔗属,9~11条来自蔗茅属斑茅,3份F_1的核型分类分别属于2B、1B、1B,染色体传递方式均为"n+n"。父本GXAS07-6-1及3份F_1材料中均未发现有染色体的交换或易位现象。甘蔗与斑割复合体杂交,蔗茅属斑茅染色体在亲子间传递过程存在丢失现象。

GISH Analysis of Intergeneric Complex between Erianthus arundinaceus and Saccharum spontaneum (GXAS07-6-1) and Its F1 Hybrids from Crosses with Sugarcane(Saccharum spp.)

GXAS07-6-1,an intergeneric hybrid of Erianthus arundinaceus(Retz.) Jeswiet and Saccharum spontaneum L.has the advantages of its parents.In this study,the turth and fales of three F1 hybrids progenies GXAS08-2-17,GXAS08-2-22 and GXAS08-2-32 were identified by the PCR method of Alu-like.The chromosomes composition and karyotype of the GXAS07-6-1 and the F1 generation were analyzed using the genomic in situ hybridization (GISH)technique.The results showed that the three F1 materials were true progenies.There were 62 chromosomes in GXAS07-6-1,of which 30 were from E.arundinaceus (Retz.)Jeswiet and 32 were from S.spontaneum L..and the somatic chromosome karyotypic was 1 B,following n + n transmission.Furthermore,the chromosomes numbers of GXASF108-2-17,GXASF108-2-22 and GXASF108-2-32 ranged from 78 to 80,of which 69-71 were from Saccharum spp.and 9 to 11 were from E.arundinaceus (Retz.) Jeswiet.The somatic chromosome karyotypes of three F1 progeny were 2B,1 B,1 B,respectively,and all of the three progenies following n + n transmission.Chromosome translocation and exchange were not observed in the intergeneric hybrid GXAS07-6-1 and its F1 hybrids as well.Chromosome missing also was confirmed from E.arundinaceus (Retz.)Jeswiet in the F1 progeny.