| Exo-1基因对端粒酶缺陷小鼠造血干细胞植入效率的影响 |
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| 引用本文： | 石桂英,陈显达,陈陟阳,鞠振宇. Exo-1基因对端粒酶缺陷小鼠造血干细胞植入效率的影响[J]. 中国实验动物学报, 2011, 19(2): 95-99,186. DOI: 10.3969/j.issn.1005-4847.2011.02.002 |
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| 作者姓名： | 石桂英 陈显达 陈陟阳 鞠振宇 |
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| 作者单位： | 中国医学科学院北京协和医学院实验动物研究所,北京,100021 |
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| 基金项目： | 国家自然基金面上项目，北京市科委科技新星计划 |
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| 摘 要： | 目的利用不同的造血干细胞移植方式,探讨Exo-1基因缺失对端粒酶基因敲除小鼠的造血干细胞植入效率的影响。方法以CD45.1小鼠的骨髓细胞或骨髓造血干细胞为供体,以端粒酶基因敲除小鼠或Exo-1基因和端粒酶基因双敲除小鼠为受体,在给予不同剂量X线照射或不照射的情况下,重复进行静脉注射全骨髓细胞或分选的骨髓造血干细胞(c-kit+、Sca-1+、lineage-,KSL),于移植后1个月取外周血,流式分析嵌合率。结果未经X线照射及1 Gy、2 Gy照射情况下,端粒酶基因缺陷受体小鼠的外周血中供体来源的细胞嵌合率较低;6 Gy照射后,供体来源的外周血细胞嵌合率仍低于50%,而且端粒酶基因缺陷受体小鼠在移植后1个月内死亡较多;3 Gy照射可形成较高嵌合率,Exo-1基因缺失对端粒酶缺陷小鼠的造血干细胞植入效率的影响不显著。结论以端粒酶缺陷小鼠作为衰老模型研究造血干细胞植入效率时,3 Gy X线照射能够有效地形成较高的外周血供体细胞的嵌合率,但是Exo-1基因没有进一步提高造血干细胞在端粒酶敲除小鼠的植入效率。
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| 关 键 词： | 端粒酶基因缺陷 骨髓移植 造血干细胞 衰老 |
Influence of Exo-1 gene on the chimerism rate in telomerase-defecient mice |
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| SHI Gui-ying,CHEN Xian-da,CHEN Zhi-ying,JU Zhen-yu. Influence of Exo-1 gene on the chimerism rate in telomerase-defecient mice[J]. Acta Laboratorium Animalis Scientia Sinica, 2011, 19(2): 95-99,186. DOI: 10.3969/j.issn.1005-4847.2011.02.002 |
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| Authors: | SHI Gui-ying CHEN Xian-da CHEN Zhi-ying JU Zhen-yu |
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| Affiliation: | SHI Gui-ying,CHEN Xian-da,CHEN Zhi-ying,JU Zhen-yu(Institute of Laboratory Animal Science,Chinese Academy of Medical Sciences & Comparative Medical Center,Peking Union Medical College,Beijing 100021,China) |
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| Abstract: | Objective To investigate the influence of Exo-1 gene deletion on the engraftment of hematopoietic stem cell(HSC) transplantation by different methods into the telomerase knockout mice.Methods Bone marrow cells or KSL((c-kit +,Sca-1 +,lineage-) cells were isolated from CD45.1 mice,and then injected into recipients,which were telomer-ase gene knockout or Exo-1 and telomerase gene double knockout mice with or without irradiation.At 1 month later,pe-ripheral blood was collected and analyzed by flow cytometry.Re... |
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| Keywords: | Telomerase gene dysfunction Bone marrow transplantation Hematopoietic stem cells(HSCs) Aging Mice |
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