| 牛卵母细胞玻璃化冷冻对基因mRNA表达量的影响 |
| |
| 引用本文： | 林嘉鹏,王静,赵云程,陈童,汪立芹,陈博,黄俊成. 牛卵母细胞玻璃化冷冻对基因mRNA表达量的影响[J]. 中国实验动物学报, 2011, 19(2): 134-139. DOI: 10.3969/j.issn.1005-4847.2011.02.011 |
| |
| 作者姓名： | 林嘉鹏 王静 赵云程 陈童 汪立芹 陈博 黄俊成 |
| |
| 作者单位： | 1. 新疆畜牧科学院农业部家畜繁育生物技术重点开放实验室,新疆畜牧科学院生物技术中心,乌鲁木齐,830000
2. 新疆畜牧科学院农业部家畜繁育生物技术重点开放实验室,新疆畜牧科学院生物技术中心,乌鲁木齐,830000;新疆农业大学农学院,乌鲁木齐,830052 |
| |
| 基金项目： | 新疆自治区自然科学基金，新疆自治区科技攻关，新疆自治区科技计划项目，国家支撑计划，国家"十一五"863计划 |
| |
| 摘 要： | 目的 将处于生发泡期(GV期)和体外成熟期(IVM)的牛卵母细胞进行玻璃化冷冻.解冻,对其卵裂率和囊胚率以及一些与发育相关基因的mRNA表达量进行评价.方法 玻璃化冷冻GV期(n=224)和IVM期(n=235)牛卵母细胞,解冻后对其进行体外培养并采用quantitative real time-PCR技术对冷冻.解冻...
|
| 关 键 词： | 玻璃化冷冻 基因表达 牛卵母细胞 发育能力 |
Vitrification of bovine oocytes affects their gene mRNA abundance |
| |
| LIN Jia-peng,WANG Jing,ZHAO Yun-cheng,CHEN Tong,WANG Li-qin,CHEN Bo,HUANG Jun-cheng. Vitrification of bovine oocytes affects their gene mRNA abundance[J]. Acta Laboratorium Animalis Scientia Sinica, 2011, 19(2): 134-139. DOI: 10.3969/j.issn.1005-4847.2011.02.011 |
| |
| Authors: | LIN Jia-peng WANG Jing ZHAO Yun-cheng CHEN Tong WANG Li-qin CHEN Bo HUANG Jun-cheng |
| |
| Affiliation: | LIN Jia-peng1,WANG Jing1,2,ZHAO Yun-cheng1,CHEN Tong1,WANG Li-qin1,CHEN Bo1,HUANG Jun-cheng1(1.Xinjiang Academy of Animal Sciences,The Key Laboratory of Livestock Reproduction & Breed Biotechnology of Ministry of Agriculture,Xinjiang Academy of Biotechnological Center,Urumqi 830000,China,2.Xinjiang Agricultural University Agronomy College,Urumqi 830052) |
| |
| Abstract: | Objective To evaluate the mRNA expression abundance of genes,which are related with embryonic development and ability to undergo successful fertilization,cleavage and blastocyst development,in the GV stage and in vitro matured(IVM) bovine oocytes.Methods The oocytes that vitrified-warmed at GV(n = 224) and IVM(n = 235) were in vitro fertilized and cultured up to blastocyst stage under standard conditions.In parallel,we analyzed the relative a-bundance of Bax,Dnmt1,Hdac1,cyclinb1,Cdc2 and Cu Zn SOD transcrip... |
| |
| Keywords: | Vitrification Gene expression Expression abundance Bovine oocyte Developmental competence |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
