| 小鼠心肌纤维化模型的制备及其胶原沉积的机制 |
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| 引用本文： | 邓玮,陈庆伟,李兴升,李桂琼,柯大智,莫显刚.小鼠心肌纤维化模型的制备及其胶原沉积的机制[J].中国实验动物学报,2011,19(2):103-106,188. |
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| 作者姓名： | 邓玮 陈庆伟 李兴升 李桂琼 柯大智 莫显刚 |
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| 作者单位： | 重庆医科大学附属第二医院老年病科,重庆,400010 |
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| 摘 要： | 目的 建立缺血性心肌纤维化小鼠模型并探讨其胶原沉积机制.方法 将BALB/c小鼠随机分为实验组和对照组,每组10只.实验组予以腹部皮下注射异丙肾上腺素50 ms/kg,每天2次,连续10 d.对照组同法注射生理盐水.对比体表心电图,45 d后处死小鼠,天狼猩红染色观察心脏Ⅰ、Ⅲ型胶原纤维含量,荧光定量PCR检测心脏基质金属蛋白酶9(MMP-9)、基质金属蛋白酶组织抑制剂一1(TIMP-1)基因表达,免疫组化染色分析心、肝、肾组织层粘连蛋白(LN)的表达.结果 实验组小鼠室性心律失常增多,心率加快(P<0.05);心脏胶原沉积较多,MMP-9、TIMP-1、LN表达上调(P<0.05),肝、肾组织LN无明显改变(P>0.05).结论 异丙肾上腺素能制备缺血性心肌纤维化小鼠模型,其机制与MMP-TIMP失衡有关.
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| 关 键 词： | 异丙肾上腺素 心肌纤维化 小鼠 动物模型 |
Establishment of a mouse model of myocardial fibrosis with isoproterenol and the mechanism of collagen deposition |
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| DENG Wei,CHEN Qing-wei,LI Xing-sheng,LI Gui-qiong,KE Da-zhi,MO Xian-gang.Establishment of a mouse model of myocardial fibrosis with isoproterenol and the mechanism of collagen deposition[J].Acta Laboratorium Animalis Scientia Sinica,2011,19(2):103-106,188. |
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| Authors: | DENG Wei CHEN Qing-wei LI Xing-sheng LI Gui-qiong KE Da-zhi MO Xian-gang |
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| Institution: | DENG Wei,CHEN Qing-wei,LI Xing-sheng,LI Gui-qiong,KE Da-zhi,MO Xian-gang(Second Affiliated Hospital of Chongqing Medical University,Chongqing 400010,China) |
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| Abstract: | Objective To establish a manipulation-easy animal model of post-ischemic myocardial fibrosis and in-vestigate the mechanism of collagen deposition.Methods Twenty BALB /c mice were randomly divided into two groups: the experimental group and control group.The mice in the experimental group were subjected to hypodermic injection with isoproterenol in a dose of 50 mg /kg.The injection was carried out twice a day for consecutive 10 days.Physiological saline was injected with the same method in the control group... |
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| Keywords: | Isoproterenol Myocardial fibrosis Mouse Animal model |
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