Reaction Medium Selection for An Enzymatic Peptide Synthesis in An Aqueous-Organic Two-Phase System |
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| Authors: | Montserrat Sarr m Gl ria Caminal Gl ria Gonz lez Josep L pez-Santí n |
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| Institution: | Montserrat Sarrà,m ,Glò,ria Caminal,Gló,ria Gonzá,lez,Josep Ló,pez-Santí,n |
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| Abstract: | Efficient development of enzymatic synthesis in two-phase systems is closely related with appropriate selection of the reaction medium (especially the solvent and phase ratio). A selection procedure based on the calculation of the theoretically allowable conversion and product concentration is presented and applied to a peptide synthesis using papain. For the synthesis of the dipeptide Boc-Gly-Phe-OMe, the operating conditions have been determined, and the two-phase system to be used has been successfully selected (with trichloroethylene being the best solvent). |
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| Keywords: | Two-phase system Reaction medium engineering Extractive biocatalysis Enzymatic peptide synthesis Papain |
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