| 肺炎克雷伯菌生物膜对小鼠巨噬细胞免疫功能的影响 |
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| 引用本文： | 王云英,鲍依稀. 肺炎克雷伯菌生物膜对小鼠巨噬细胞免疫功能的影响[J]. 中国微生态学杂志, 2010, 22(2): 122-125,129 |
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| 作者姓名： | 王云英 鲍依稀 |
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| 作者单位： | 重庆医科大学附属第二医院,检验科,重庆,400010 |
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| 基金项目： | 重庆市卫生局医学科学技术研究项目 |
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| 摘 要： | 目的研究肺炎克雷伯菌生物膜(BF)对小鼠腹腔巨噬细胞TLRs mRNA和细胞因子表达的影响,探索机体抗BF感染免疫的特点。方法将雄性昆明种小鼠40只随机分成2组,一组腹腔植入体外形成肺炎克雷伯菌BF的硅胶片,建立留置性医疗装置BF感染模型实验组,另一组植入与实验组同等量的浮游菌作为对照组。实时定量PCR分析2组巨噬细胞TLRs mRNA的表达水平,双抗体夹心ELISA法测定细胞因子的含量。结果实验BF组巨噬细胞TLR2、TLR4 mRNA表达量是对照浮游菌组的0.23和0.24倍;而TLR5、TLR9两组表达差异无显著性。实验BF组刺激前后IL-1、IL-2的差值明显低于对照浮游菌组,而IL-4则相反(P0.01)。结论与浮游菌相比,BF能下调小鼠腹腔巨噬细胞TLR2、TLR4的表达,机体的免疫应答朝着Th2型免疫反应发展,这可能是BF相对浮游菌更容易逃脱机体免疫防御系统、引起慢性感染的机制之一。
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| 关 键 词： | 肺炎克雷伯菌生物膜 巨噬细胞 TLRs受体mRNA 细胞因子 |
Influence of Klebsiella pneumoniae biofilm on immunological function in mouse macrophage |
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| WANG Yun-ying,BAO Yi-xi. Influence of Klebsiella pneumoniae biofilm on immunological function in mouse macrophage[J]. Chinese Journal of Microecology, 2010, 22(2): 122-125,129 |
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| Authors: | WANG Yun-ying BAO Yi-xi |
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| Affiliation: | WANG Yun-ying,BAO Yi-xi(Department of Clinical Laboratory,the Second Affiliated Hospital of Chongqing Medical University,Chongqing 400010,China) |
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| Abstract: | Objective To study the influence of Klebsiella pneumoniae biofilm on the expressions of Toll like receptors mRNA and Cytokines and explore the mechanismof anti-bacterial biofilm in mouse peritoneal macrophage.Method Forty kunming mice were randomly divided into experiment group and control group.The mRNA expression level of TLRs was detected by rea-time polymerase chain reaction.Plasma Cytokines levels were measured by double antibody enzyme linked-immunosorbent assay.Result The TLR2 and TLR4 mRNA expressio... |
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| Keywords: | Klebsiella pneumoniae biofilm Peritoneal macrophage Toll like receptors mRNA Cytokines |
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