Role of silicon in diatom metabolism. VII. Silicic acid-stimulated DNA synthesis in toluene-permeabilized cells of Cylindrotheca fusiformis. |
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Authors: | C W Sullivan B E Volcani |
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Institution: | Laboratory of Cellular and Comparative Physiology, Gerontology Research Center, National Institute on Aging, National Institutes of Health, USPHS, Department of Health, Education and Welfare, Baltimore, MD 21224, USA |
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Abstract: | Human diploid cell populations were fractionated on the basis of cell size by gravity sedimentation. This cell separation procedure yielded fractionated cell populations that were enriched for both large cell volumes and for slow and/or non-replicating cells (cells which did not have labeled nuclei after a 48 h incubation with 3H]TdR). It also yielded fractionated cell populations that were enriched for small cell volumes and for rapidly replicating cells (cells with labeled nuclei). However, upon reintroduction to tissue culture conditions, cell populations lost their fractionated properties and soon resembled unfractionated cell cultures at similar levels of in vitro passage. |
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Keywords: | To whom reprint requests should be addressed |
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