SRAP分子标记分析西瓜遗传多态性

目的:探讨西瓜遗传多样性和遗传基础。方法:采用SRAP分子标记对西瓜品种D1、D2、D3、H1、H2、H3、M1、M2、M3、m1、m2、m3的多态性进行了分析。结果:每对引物组合产生13~25对比较清晰的扩增带.8对引物组合共产生131条扩增带。平均每对引物组合产生16.375条。8对引物组合共产生多态性带37条,每对引物组合产生3~7条,平均4.625条。每对引物组合产生的多态性带的比例为16.666%~38.464%,平均为28.675%。另外,对银染过程进行了优化。结论:SRAP标记多态性还是较高的,可以适于分析西瓜等遗传差异小的作物。

Genetic Polymorphism Analysis by Sequence-related Amplified Polymorphism Markers in Citrullus lanatus

Objective:In order to investigate the Genetic diversity and genetic basis of Citrullus lanatus.Methods:Polymorphism among D1,D2,D3,H1,H2,H3,M1,M2,M3,m1,m2,m3 were analyzed with SRAP markers.Results:There were 13-25 clear combination bands per primer.About 131 amplified fragments and 37 polymorphic fragments were detected 8 SRAP primer combinations.There were 3-7 clear polymorphic combination bands per primer. The number of amplified fragments and polymorphic fragments per primer combination were 16.375 and 4.625 respectively.The percentage of polymorphic fragments was 16.666%-38.464%,average 28.675%. In addtion,optimizated the reaction of silver dyeing.Conclusion:All these results showed that the Polymorphism of SRAP markers is highely,and could be widely used in analyzed crops such as citrullus lanatus which has small Genetic Difference.