Genetic features of a pollen-part mutation suggest an inhibitory role for the <Emphasis Type="Italic">Antirrhinum</Emphasis> pollen self-incompatibility determinant |
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Authors: | Yongbiao Xue Yijing Zhang Qiuying Yang Qun Li Zhukuan Cheng Hugh G Dickinson |
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Institution: | (1) Laboratory of Molecular and Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences and National Center for Plant Gene Research, West Lincui Road, 100101 Chaoyang District, Beijing, China;(2) The State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences and National Center for Plant Gene Research, 100101 Beijing, China;(3) Department of Plant Sciences, University of Oxford, South Parks Road, Oxford, OX1 3RB, UK;(4) Graduate University of Chinese Academy of Sciences, Beijing, 100049, China |
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Abstract: | Self-incompatibility (SI), an important barrier to inbreeding in flowering plants, is controlled in many species by a single
polymorphic S-locus. In the Solanaceae, two tightly linked S-locus genes, S-RNase and SLF (S-locus F-box)/SFB (S-haplotype-specific F-box), control SI expression in pistil and pollen, respectively. The pollen S-determinant appears to function to inhibit all but self S-RNase in the Solanaceae, but its genetic function in the closely-related
Plantaginaceae remains equivocal. We have employed transposon mutagenesis in a member of the Plantaginaceae (Antirrhinum) to generate a pollen-part SI-breakdown mutant Pma1 (Pollen-part mutation in Antirrhinum1). Molecular genetic analyses showed that an extra telocentric chromosome containing AhSLF-S
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is present in its self-compatible but not in its SI progeny. Furthermore, analysis of the effects of selection revealed positive
selection acting on both SLFs and SFBs, but with a stronger purifying selection on SLFs. Taken together, our results suggest an inhibitor role of the pollen S in the Plantaginaceae (as represented by Antirrhinum), similar to that found in the Solanaceae. The implication of these findings is discussed in the context of S-locus evolution in flowering plants.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
Yongbiao Xue, Yijing Zhang, and Qiuying Yang contributed equally to this work. |
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Keywords: | Self-incompatibility Antirrhinum SLF Pollen-part mutation S-RNase |
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