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Isolation and Characterization of Autoreactive T Cells in Experimental Autoimmune Encephalomyelitis of the Mouse |
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| Authors: | Stefan Brocke Laura Quigley Henry F. McFarland Lawrence Steinman |
| Affiliation: | aDepartment of Neurology and Neurological Sciences, Stanford University Medical Center, Stanford, California, 94305;bNational Institute of Neurological Disorders and Stroke, National Institutes of Health, Building 10, Room 5B-16, 10 Center Drive, MSC 1400, Bethesda, Maryland, 20892-1400;cWeizmann Institute of Science, 76100, Rehovot, Israel |
| Abstract: | Experimental autoimmune encephalomyelitis (EAE) can be adoptively transferred by T cells that are specific for autoantigens of the central nervous system. A variety of autoantigens and their derived peptides have been shown to be excellent stimulators for encephalitogenic T-cell lines and clones. This article describes protocols for the establishment and characterization of autoreactive T-cell lines and clones and for the study of EAE induced by these cells. The rationale for using transferred EAE models is discussed, together with advantages and disadvantages of usingin vitrocultured T cells compared with Freund's adjuvant-based immunization for the induction of EAE. |
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