Toxic effect of alantolactone and dihydroalantolactone in in vitro cultures of leukocytes. |
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Authors: | G Dupuis J Brisson |
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Affiliation: | Department of Biochemistry, Centre Hospitalier Universitaire, University of Sherbrooke, Sherbrooke, J1H 5N4 Québec Canada |
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Abstract: | Alantolactone, an allergenic sesquiterpene lactone, is toxic to leukocytes in in vitro cultures. Cell (1 X 10(6) cells/ml of culture) stimulation by pokeweed mitogen (PWM) decreases with increasing amounts of terpene. A concentration of 1 mug/ml of culture decreases stimulation by 50%. The reduced terpene, dihydro-11,13-alantolactone (DHA) is also toxic. A concentration of 1.7 mug/ml of culture of DHA brings about a 50% decrease in stimulation. Both compounds affect cell viability as measured by dye exclusion. It is suggested that the toxicity of alantolactone is not due to the presence of the alpha-methylene group conjugated to the carbonyl function of the gamma-lactone system. |
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Keywords: | ACD allergic contact dermatitis DHA dihydro-11,13-alantolactone NCS Nuclear Chicago Solubilizer PHA phytohemagglutinin PWA pokeweed mitogen TCA trichloroacetic acid |
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