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Application of 13C mineral carbon for assessment of the primary production of organic matter in aquatic environments
Authors:N. V. Pimenov  A. M. Zyakun  T. S. Prusakova  O. N. Lunina  M. V. Ivanov
Affiliation:(1) Winogradsky Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 2, Moscow, 117312, Russia;(2) Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, Pushchino, Moscow oblast, 142290, Russia
Abstract:The possibility of measuring the rates of light and dark CO2 assimilation using 13C carbonate was demonstrated on Lake Kichier (Marii El). The application of methods utilizing the stable 13C and the radioactive 14C isotopes resulted in comparable values of the rates of light and dark CO2 fixation. Due to its absolute environmental safety, the method with 13C mineral carbon can be recommended as an alternative to radioisotope methods for qualitative measurements of CO2 fixation rates in aquatic ecosystems.
Keywords:primary production  meromictic lakes   13C stable isotope method   14C radioisotope method  anoxygenic phototrophs
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