烤烟根系分泌物中单糖的组分含量及其相关分析

以4个不同烤烟品种为研究材料,采用盆栽试验,运用高效毛细管区带电泳法测定各品种根际土、非根际土、根系及叶片中的单糖组分及含量，并分析其相关关系，探究根系分泌物中糖类的分泌特性。结果表明：在各样品中,共检出木糖、葡萄糖、半乳糖、核糖、阿拉伯糖和鼠李糖6种糖;不同品种根际土、非根际土、根系及叶片中检出的糖组分及含量均存在差异;同一品种中,叶片最高,根系次之,根际土和非根际土最低;相关性分析表明,木糖、阿拉伯糖、葡萄糖、鼠李糖和半乳糖总量在根际土、非根际土、叶片和根系间呈正相关关系,各单糖组分间均呈正相关关系,部分组分呈显著或极显著相关关系。研究表明，不同烤烟品种根系分泌这些单糖存在品种差异,且根系分泌单糖可能是一个沿浓度梯度的扩散过程。

Correlation Analysis of Monosaccharide Composition and Contents in Flue-cured Tobacco Root Exudates

In order to explore the monosaccharide composition and contents in root exudates and plant tissue,four flue-cured tobacco varieties were used as the experimental material.The composition and contents of monosaccharide in rhizosphere,non-rhizosphere soils,root and leaves of each variety were analyzed with high performance capillary electrophoresis in pot experiment.Six monosaccharides analyzed in all of varieties,they were xylose,glucose,galactose,ribose,arabinose,and rhamose.The composition and contents of monosaccharide were different in rhizosphere soils,non-rhizosphere soils,root and leaves.In the same variety,leaves have the highest content,root takes second place,and rhizosphere,non-rhizosphere soils are the least.The correlation analysis show that there was a positive correlation between rhizosphere soils,non-rhizosphere soils,leaves and roots in total contents of xylose,arabinose,glucose,rhamnose and galactose.There was also a significant positive correlation between different monosaccharide for some of them.The results indicated that monosaccharides of root were secreted along with concentration gradient and diffused into rhizosphere.