T-DNA transfer to maize plants |
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| Authors: | Wen-Hui Shen Jesús Escudero Barbara Hohn |
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| Institution: | (1) IBMP/CNRS, 12 rue du Général Zimmer, 67084 Strasbourg, France |
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| Abstract: | Agrobacterium-mediated transformation is the method of choice to engineer desirable genes into plants. Here we describe a protocol for
demonstrating T-DNA transfer from Agrobacterium into the economically important graminaceous plant maize. Expression of the T-DNA-located GUS gene was observed with high
efficiency on shoots of young maize seedlings after cocultivation with Agrobacterium. |
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| Keywords: | Agrobacterium tumefaciens Zea mays β -glucuronidase (GUS) T-DNA transfer |
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